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Methods of structural analysis A
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Methods of structural analysis A
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Anno accademico 2021/2022
- Codice dell'attività didattica
- BIO0198A
- Docente
- Prof. Giuseppe Spoto (Titolare del corso)
- Insegnamento integrato
- METHODS OF STRUCTURAL ANALYSIS (BIO0198)
- Corso di studi
- Laurea Triennale in Biotecnologie
- Anno
- 3° anno
- Periodo didattico
- Secondo semestre
- Tipologia
- A scelta dello studente
- Crediti/Valenza
- 3
- SSD dell'attività didattica
- CHIM/02 - chimica fisica
- Modalità di erogazione
- Tradizionale
- Lingua di insegnamento
- Inglese
- Modalità di frequenza
- Facoltativa
- Tipologia d'esame
- Scritto ed orale
- Prerequisiti
- Basic knowledge of Inorganic Chemistry, Organic Chemistry, Physical Chemistry, Biochemistry.
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Sommario insegnamento
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Obiettivi formativi
The integrated course is aimed at providing students with the knowledge necessary to understand how the structure of biomolecules (mainly proteins and nucleic acids) is determined by spectroscopic and diffractometric methods, knowing how to identify which method(s) to use according to the characteristics of the system under study, and critically interpret the three-dimensional structure.
In this module (A) the basis of optical (vibrational and electronic) spectroscopies are treated in some detail and their applications in the biological field described.
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Risultati dell'apprendimento attesi
Modalità di esame.
Traditionally the exam consists of a written test and an oral test. The written test includes the answer to questions (both multiple choice and open) concerning biomolecular and X-ray NMR spectroscopy. The oral exam focus on the part of IR vibrational spectroscopy and electronic spectroscopy.
In case of need for confinement and/or total or partial lockdown the exam will be in live straeming on the Webex platform.
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Modalità di insegnamento
Traditionally, the course consists of frontal lectures on the blackboard with the projection of slides to support the topics covered.
If the current needs for confinement persist and in the event of total or partial lockdown the course will be delivered electronically (synchronous lessons in live streaming, recorded and published on the Webex platform).
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Modalità di verifica dell'apprendimento
Traditionally, the exam is oral and in presence.
If the current needs for confinement persist and in the event of total or partial lockdown, the exam will be in live streaming on the Webex platform.
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Programma
A Module: Vibrational and electronic spectroscopy (3 CFU).
This part will deal with the principles of IR vibrational spectroscopy and electronic absorption and emission (in steady state and resolved over time) and the applications of these methodologies to the study of biomolecules in different conditions (solid state, solution). In particular, the possibility of using IR spectroscopy to obtain information on the secondary and tertiary structure of proteins and the secondary structure of nucleic acids and to monitor their possible change due to interactions and / or chemical and physical modifications of the surroundings will be discussed. As for electronic spectroscopy, the aspects of quantitative sensitivity, the main types of signals sensitive to the structural organization of bio-macromolecules and metal centers possibly present, and the possibility of obtaining information on the structure and its possible modifications through the evolution of the characteristics of fluorescence signals are illustrated.
Testi consigliati e bibliografia
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The teacher provides in advance the slides used during the lesson, which are a good starting material for the study.
The slides also includes many references to papers available in the scientific literature as well as links to specialized sites and other useful materials available in internet.
Specialistic reference books:
Colthup e L.H. Daly, Introduction to infrared and Raman spectroscopy, Academic Press, 1975,New York
J.M. Chalmers e P.R. Griffits (eds), Handvbook of Vibrational Spectroscopy, Wiley,Chichester, 2002, Vol. 5.
I.D. Campbell e R. A. Dwek, Biological Spectroscopy, "Ultraviolet and Visible Absoprtion spectroscopy" (Cap. 4), e " Fluorescence" (Cap. 5), The Benjamin/Cummings Publishing Company, Menlo Park,1984.
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Note
“Le modalità di svolgimento dell'attività didattica potranno subire variazioni in base alle limitazioni imposte dalla crisi sanitaria in corso. In ogni caso è assicurata la modalità a distanza per tutto l'anno accademico”
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