Corso di Laurea in Biotecnologie

BIOCHEMICAL, MOLECULAR AND CELLULAR TECHNOLOGIES

BIOCHEMICAL, MOLECULAR AND CELLULAR TECHNOLOGIES

Anno accademico 2020/2021

Sommario insegnamento

• Obiettivi formativi
• Risultati dell'apprendimento attesi
• Modalità di insegnamento
• Modalità di verifica dell'apprendimento
• Attività di supporto
• Programma
• Testi consigliati e bibliografia
• Note
• Moduli didattici
• Strumenti didattici

Obiettivi formativi

Biochemistry and Cellular Technology Module:

The purpose of the biochemical methods is to give students the theoretical and practical knowledge of the most important methods of biological laboratory: cell culture, cell viabilty and proliferation assay, extraction and purification of nucleci acids and proteins, functional and biochemical characterization of proteins.

Molecular Biology Module:

The purpose of the Molecular Biology Module is to give students the theoretical and practical knowledge of the most important methods in DNA/RNA sequencing and bioinformatics data analysis

Risultati dell'apprendimento attesi

Biochemistry and Molecular Biology Module:

The student should be able to navigate through procedures (and be aware of the limitations of them)

Modalità di insegnamento

Biochemistry and Molecular Biology Module:

Lessons and practical laboratory activities

N.B. To access to lessons and other materialyou must regsiter to the Moodle of the course:

https://biotec.i-learn.unito.it/course/view.php?id=405

Modalità di verifica dell'apprendimento

Biochemistry and Molecular Biology Module:

Writed text
Open and closed questions

Attività di supporto

Biochemistry and Molecular Biology Module:

Practical laboratory for the biochemistry method: 16 hours

Practical Next Generation Sequencing Data Analysis: 16 hours

Programma

Biochemistry and Cellular Technology Module

The following techniques will be treated in the module:

• Sterile cell culture technique, propagation of 2D cell culture
• 3D cell cultures
• Assays of cell viability and proliferation.
• Isolation and purification of biological moleucles from cells and tissues
• Electrophoresis techniques for purity check. PAGE, IEF and 2D electrophoresis for proteomics.
• Protein blotting and decoration 
• Immunofluorescence and confocal microscopy
• Lab practicals: Recombinant protein expression systems. purification of a GST-tag fusion protein, enzyme activity assay by spectrophotometric methods. SDS-PAGE, western blotting, protein quantitation and discussion on characterisation techniques by spectrophotometric advanced methods.

Molecular Biology Module

The Molecular Biology Module will focus on second and third generation DNA sequencing technologies.
The module focuses on experimental and computational aspects of DNA/RNA sequencing.
Introduction to sequencing technologies:

• Illumina (HISeq, MiSeq), Roche (454), Life Technologies (Ion Torrent).

Sample preparation:

• miRNA-seq
• mRNA seq
• whole transcriptome sequencing
• directional sequencing
• enrichment protocols
• DNA exome
• DNA whole genome

Bioinformatics analysis approaches

• Mapping
• Filtering
• Differential expression
• Single cell data analysis

Examples of the above mentioned technologies and analytical methods
Molecular Biology Module: theoretical lessons 8 hours, data analysis 16 hours

Biochemistry

Rob Reed, David Holmes, Jonathan Weyers, Allan Jones Metodologie di base per le scienze biomolecolari, Zanichelli (2002).

or

A.J. Ninfa, D.P. Ballou. Metodologie di base per la biochimica e la biotecnologia. Zanichelli (2000).

or

IC Wilson, J. Walker. Metodologia biochimica: le tecniche biochimiche in laboratorio. Ed. Raffaello Cortina (1995).

Molecular Biology Module:

RNA-seq Data Analysis: A Practical Approach
Autore: Eija Korpelainen, Jarno Tuimala, Panu Somervuo, Mikael Huss, Garry WongEdizione: 1st Edition
Casa editrice: Chapman & Hall/CRC Mathematical and Computational Biology
ISBN: 9781466595002
Url: https://www.crcpress.com/RNA-seq-Data-Analysis-A-Practical-Approach/Korpelainen-Tuimala-Somervuo-Huss-Wong/p/book/9781466595002

Orario lezioni

Note

“Le modalità di svolgimento dell'attività didattica potranno subire variazioni in base alle limitazioni imposte dalla crisi sanitaria in corso. In ogni caso è assicurata la modalità a distanza per tutto l'anno accademico”

Lessons ppt and recording will be available at:

https://biotec.i-learn.unito.it/course/view.php?id=405

Moduli didattici

• Biochemical, molecular and cellular technologies A (BIO0201A)
• Biochemical, molecular and cellular technologies B (BIO0201B)

• Materiale didattico
• Bacheca appelli
• Vai a Moodle
• Visita i forum