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BIOCHEMICAL, MOLECULAR AND CELLULAR TECHNOLOGIES
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BIOCHEMICAL, MOLECULAR AND CELLULAR TECHNOLOGIES
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Anno accademico 2020/2021
- Codice dell'attività didattica
- BIO0201
- Docenti
- Prof. Luca Primo (Titolare del corso)
Prof. Raffaele Adolfo Calogero (Titolare del corso) - Corso di studi
- Laurea Triennale in Biotecnologie
- Anno
- 3° anno
- Periodo didattico
- Da definire
- Tipologia
- A scelta dello studente
- Crediti/Valenza
- 6
- SSD dell'attività didattica
- BIO/10 - biochimica
BIO/11 - biologia molecolare - Modalità di erogazione
- Tradizionale
- Lingua di insegnamento
- Inglese
- Modalità di frequenza
- Facoltativa
- Tipologia d'esame
- Scritto
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Sommario insegnamento
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Obiettivi formativi
Biochemistry and Cellular Technology Module:
The purpose of the biochemical methods is to give students the theoretical and practical knowledge of the most important methods of biological laboratory: cell culture, cell viabilty and proliferation assay, extraction and purification of nucleci acids and proteins, functional and biochemical characterization of proteins.
Molecular Biology Module:
The purpose of the Molecular Biology Module is to give students the theoretical and practical knowledge of the most important methods in DNA/RNA sequencing and bioinformatics data analysis
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Risultati dell'apprendimento attesi
Biochemistry and Molecular Biology Module:
The student should be able to navigate through procedures (and be aware of the limitations of them)
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Modalità di insegnamento
Biochemistry and Molecular Biology Module:
Lessons and practical laboratory activities
N.B. To access to lessons and other materialyou must regsiter to the Moodle of the course:
https://biotec.i-learn.unito.it/course/view.php?id=405
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Modalità di verifica dell'apprendimento
Biochemistry and Molecular Biology Module:
Writed text
Open and closed questions- Oggetto:
Attività di supporto
Biochemistry and Molecular Biology Module:
Practical laboratory for the biochemistry method: 16 hours
Practical Next Generation Sequencing Data Analysis: 16 hours
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Programma
Biochemistry and Cellular Technology Module
The following techniques will be treated in the module:
- Sterile cell culture technique, propagation of 2D cell culture
- 3D cell cultures
- Assays of cell viability and proliferation.
- Isolation and purification of biological moleucles from cells and tissues
- Electrophoresis techniques for purity check. PAGE, IEF and 2D electrophoresis for proteomics.
- Protein blotting and decoration
- Immunofluorescence and confocal microscopy
- Lab practicals: Recombinant protein expression systems. purification of a GST-tag fusion protein, enzyme activity assay by spectrophotometric methods. SDS-PAGE, western blotting, protein quantitation and discussion on characterisation techniques by spectrophotometric advanced methods.
Molecular Biology Module
The Molecular Biology Module will focus on second and third generation DNA sequencing technologies.
The module focuses on experimental and computational aspects of DNA/RNA sequencing.
Introduction to sequencing technologies:- Illumina (HISeq, MiSeq), Roche (454), Life Technologies (Ion Torrent).
Sample preparation:
- miRNA-seq
- mRNA seq
- whole transcriptome sequencing
- directional sequencing
- enrichment protocols
- DNA exome
- DNA whole genome
Bioinformatics analysis approaches
- Mapping
- Filtering
- Differential expression
- Single cell data analysis
Examples of the above mentioned technologies and analytical methods
Molecular Biology Module: theoretical lessons 8 hours, data analysis 16 hoursTesti consigliati e bibliografia
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Biochemistry
Rob Reed, David Holmes, Jonathan Weyers, Allan Jones Metodologie di base per le scienze biomolecolari, Zanichelli (2002).
or
A.J. Ninfa, D.P. Ballou. Metodologie di base per la biochimica e la biotecnologia. Zanichelli (2000).
or
IC Wilson, J. Walker. Metodologia biochimica: le tecniche biochimiche in laboratorio. Ed. Raffaello Cortina (1995).
Molecular Biology Module:
RNA-seq Data Analysis: A Practical Approach
Autore: Eija Korpelainen, Jarno Tuimala, Panu Somervuo, Mikael Huss, Garry WongEdizione: 1st Edition
Casa editrice: Chapman & Hall/CRC Mathematical and Computational Biology
ISBN: 9781466595002
Url: https://www.crcpress.com/RNA-seq-Data-Analysis-A-Practical-Approach/Korpelainen-Tuimala-Somervuo-Huss-Wong/p/book/9781466595002- Oggetto:
Orario lezioni
Lezioni: dal 15/10/2020 al 15/01/2021
Nota: IMPORTANT: Because there are overlaps with other courses the lesson on Friday will be rescheduled on the basis of time availability of the students.
Lessons will be provided on-line using webex platform.
The list of the links to access to the webex instances will be provided in the moodle of the course.
Link to the moodle will be loaded here in few days.
Lessons time:
Thursday 11-13
Friday 14-17Prof. Calogero lessons:
15/10/2020 (Joined with Prof. Primo for course presentation)
16/10/2020
23/10/2020
29/10/2020
30/10/2020
06/11/2020
13/11/2020
20/11/2020
27/11/2020
04/12/2020
11/12/2020
18/12/2020Lessons Prof. Primo
22/10/2020
05/11/2020
12/11/2020
19/11/2020Other 4 practical lessons will be defined on the basis of didactic lab availability
First lesson: 15/10/2020 14:00
Meeting link:
https://unito.webex.com/unito-en/j.php?MTID=m22ef793406e0f5f8379244762f2edf8b
Meeting number:
121 625 6158
Password:
m86pGkw5UkNSecond Lesson: 16/10/2020 14:00
https://unito.webex.com/unito-en/j.php?MTID=mbfcb123f63867d28675731bb8bcd0097
Meeting number (access code): 121 425 7096
Meeting password: xjPTnAWb287- Oggetto:
Note
“Le modalità di svolgimento dell'attività didattica potranno subire variazioni in base alle limitazioni imposte dalla crisi sanitaria in corso. In ogni caso è assicurata la modalità a distanza per tutto l'anno accademico”
Lessons ppt and recording will be available at:
https://biotec.i-learn.unito.it/course/view.php?id=405
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Moduli didattici
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